[1]乔中全,王晓明,陈建军,等.适用于转录组测序的灰毡毛忍冬体胚总 RNA提取方法筛选[J].湖南林业科技,2015,(06):99-101.[doi:10.3969/j.issn.1003-5710.2015.06.019]
 ,,et al.Screening of methods to extract total RNA from microspore of somatic embryos of Lonicera macranthoides HandMazz for transcriptome sequencing[J].Hunan Forestry Science & Technology,2015,(06):99-101.[doi:10.3969/j.issn.1003-5710.2015.06.019]
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适用于转录组测序的灰毡毛忍冬体胚总 RNA提取方法筛选()
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《湖南林业科技》[ISSN:1003-5710/CN:43-1096/S]

卷:
期数:
2015年06期
页码:
99-101
栏目:
研究报告
出版日期:
2015-11-30

文章信息/Info

Title:
Screening of methods to extract total RNA from microspore of somatic embryos of Lonicera macranthoides HandMazz for transcriptome sequencing
文章编号:
1003-5710(2015)06-0099-03
作者:
乔中全;? 王晓明;? 陈建军;? 曾慧杰;? 李永欣;? 蔡能
湖南省林业科学院, 湖南 长沙410004
Author(s):
QIAOZhongquan;?CHENJianjun;?WANGXiaoming;? ZENGHuijie;?LIYongxin;?CAINeng
HunanAcademyofForestry,Changsha410004,China
关键词:
灰毡毛忍冬;? 体胚;? RNA提取;? 转录组测序
Keywords:
Lonicera macranthoides HandMazz;? somatic embryos;? RNA extraction;? transcriptome sequencing
分类号:
Q 9497812
DOI:
10.3969/j.issn.1003-5710.2015.06.019
文献标志码:
A
摘要:
为了找到适用于转录组测序要求的灰毡毛忍冬体胚总 RNA 的提取方法,以体胚发育过程中的球形胚、心形胚、鱼雷胚和子叶胚为实验材料,比较分析了EASYspin Plus植物RNA快速提取试剂盒法、RNA Cleanup Kit试剂盒法及改良 CTAB 法3种方法的 RNA 提取效果。结果表明: 改良 CTAB 法提取的RNA浓度较低,提取过程中发生降解;RNA Cleanup Kit试剂盒法提取的RNA完整性较差,RIN值没能达到转录组测序的要求。EASYspin Plus植物RNA快速提取试剂盒法提取的RNA质量最高,且完整性好,能够满足转录组测序的要求。
Abstract:
In order to find out microspore extracting method suitable for transcriptome sequencing,this study took somatic embryos of Lonicera macranthoides HandMazz as experimental material and microspore induced with globular embryo, heart shaped embryo, torpedo embryo and embryo as research object This study compared and analyzed the RNA extraction effects by 3 different methods:EASYspin Plus method, RNA Cleanup Kit method, improved CTAB method The results showed that RNA concentrations extracted by improved CTAB method were lower,and degradationappearedinextraction process Integrity of RNA extracted by RNA Cleanup Kit method was bad,and the RIN value did not meet the requirements of transcriptome sequencing.Quality of RNA extracted by EASYspin Plus method was the highest,and the RNA integrity was also good.Therefore,RNA obtained by this method was suitable for satisfying the requirement of transcriptome sequencing.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:湖南省科学技术厅科技计划重点项目:金银花体胚发生的分子基础与再生研究(2012FJ2011)。
作者简介:乔中全(1985-),男,山东省曹县人,研究实习员,主要从事林木遗传育种、栽培及生物技术研究。
更新日期/Last Update: 2015-11-30